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KMID : 0613820100200111634
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Journal of Life Science
2010 Volume.20 No. 11 p.1634 ~ p.1639
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Induction of Physiological Sex-Reversal and Gynogenetic Diploid in Rainbow Trout, Oncorhynchus mykiss
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Lee Cheul-Ho
Kim Dae-Jung
Jeong Chang-Hwa
CHoi Gyeong-Cheol
Lee Chae-Sung
Kim Dong-Soo
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Abstract
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This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 ㎎ of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at 10℃ and 13℃. More than 93% of the male population was produced by this method and growth of hormone-treated fish at 13℃ was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least 3,600 erg/㎠ was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at 28℃ for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.
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KEYWORD
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Rainbow trout, sex-reversal, gynogenetic diploid, 17 alpha-methyltestosterone
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